ApexBio/(-)-Epigallocatechin gallate (EGCG)/500mg/A2600,ApexBio,,ApexBio,apexbt,+,,Array.Array蚂蚁淘厂家直采.

产品名称：ApexBio/(-)-Epigallocatechin gallate (EGCG)/500mg/A2600

产品编号：A2600

市场价：¥4200.00

场地：美国(厂家直采)

产品分类：蛋白类>聚合物>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$210.00

品牌： ApexBio

公司：ApexBio

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商品介绍

Size	Price	Stock
10mM (in 1mL DMSO)	$55.00	In stock
Evaluation Sample	$28.00	In stock
100mg	$50.00	In stock
500mg	$210.00	In stock

Publications citing ApexBio Products

Nature.2017 Jan 19;541(7637):417-420.

Nature.2018 Nov;563(7731):407-411.

Nature.2018 Jun 13.

Nature.2018 Jun 27.

Nature.2018 Mar 29;555(7698):673-677.

Nature.2017 Sep 7;549(7670):96-100.

Nature.2016 Apr 21;532(7599):398-401.

Science.2016 Aug 5;353(6299)594-8

Nat Nanotechnol.2017 Dec;12(12):1190-1198.

Nature Biotechnology.2017 Jun;35(6):569-576

Nat Med.2018 Sep 17.

Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.

Cell.Available online 25 October 2018.

Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.

Cell.2018 Jun 28;174(1):172-186.e21.

Cell.2018 Feb 22;172(5):1007-1021.e17.

Cell.2017 Nov 30;171(6):1284-1300.e21.

Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.

Cell.2017 Jul 13;170(2):312-323

Nat Med.2018 Jan 29.

Nat Med.2017 Nov;23(11):1342-1351.

Cell.2017 Apr 6;169(2):286-300.

Cell.2015 Aug 27;162(5):987-1002.

Cell.2015 Feb 12;160(4):729-44.

Nature Medicine.2017 Apr;23(4):493-500.

Cancer Cell.2018 May 14;33(5):905-921.e5.

Cancer Cell.2018 Apr 9;33(4):752-769.e8.

Cancer Cell.2018 Mar 12;33(3):401-416.e8.

Cancer Cell.2017 Aug 14;32(2):253-267.e5.

Nat Methods.2018 Jul;15(7):523-526.

Cell Stem Cell.2018 May 3;22(5):769-778.e4.

Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.

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Quality Control

Quality Control & MSDS

View current batch:

Purity = 99.12%

COA (Certificate Of Analysis)
HPLC
NMR (Nuclear Magnetic Resonance)
MSDS (Material Safety Data Sheet)
Datasheet

Chemical structure

Biological Activity

Description	(-)-Epigallocatechin Gallate is the main catechin extraction of green tea.
Targets	PKC
IC50

Protocol

Cell experiment [1]:
Cell lines	Human and rat neural progenitor cells (NPCs)
Preparation method	The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reaction Conditions	0, 1, 2, 5 and 10 μM; 24 or 48 hrs
Applications	(-)-Epigallocatechin Gallate (EGCG) altered human and rat NPC development in vitro. EGCG affected migration distance, migration pattern and nuclear density of NPCs growing as neurospheres. EGCG exerted these functional impairments by binding to the extracellular matrix (ECM) glycoprotein laminin, preventing its binding to β1-integrin subunits, thereby prohibiting cell adhesion and resulting in altered glia alignment and decreased number of migrating young neurons.
Animal experiment [2]:
Animal models	A rat model of partial bladder outlet obstruction (pBOO)-induced bladder injury
Dosage form	4.5 mg/kg/day; i.p.; 2 days or 30 days
Applications	EGCG attenuated bladder inflammation caused by pBOO at the 48th hr. At the 30th day, EGCG attenuated endoplasmic reticulum (ER) stress-related apoptosis. In addition, EGCG improved bladder compliance, contractile frequency and inflammation at the 30th day.
Other notes	Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References: [1]. Barenys M, Gassmann K, Baksmeier C, Heinz S, Reverte I, Schmuck M, Temme T, Bendt F, Zschauer TC, Rockel TD, Unfried K, W?tjen W, Sundaram SM, Heuer H, Colomina MT, Fritsche E. Epigallocatechin gallate (EGCG) inhibits adhesion and migration of neural progenitor cells in vitro. Arch Toxicol. 2016 Apr 26. [2]. Hsieh JT, Kuo KL, Liu SH, Shi CS, Chang HC, Lin WC, Chou CT, Hsu CH, Liao SM, Wang ZH, Li CC, Huang KH. Epigallocatechin Gallate Attenuates Partial Bladder Outlet Obstruction-induced Bladder Injury via Suppression of Endoplasmic Reticulum Stress-related Apoptosis-In Vivo Study. Urology. 2016 May;91:242.e1-9.

(-)-Epigallocatechin gallate (EGCG) Dilution Calculator

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(-)-Epigallocatechin gallate (EGCG) Molarity Calculator

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Chemical Properties

Cas No.	989-51-5	SDF	Download SDF
Synonyms	EGCG
Chemical Name	[(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate
Canonical SMILES	C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O
Formula	C₂₂H₁₈O₁₁	M.Wt	458.37
Solubility	≥22.9mg/mL in DMSO	Storage	Store at -20°C
Physical Appearance	A solid	Shipping Condition	Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Research Update

1. (-)-Epigallocatechin-gallate (EGCG) stabilize the mitochondrial enzymes and inhibits the apoptosis in cigarette smoke-induced myocardial dysfunction in rats. Mol Biol Rep. 2013 Dec;40(12):6533-45. doi: 10.1007/s11033-013-2673-5. Epub2013 Nov 7.Abstract EGCG prevents cardiac mitochondrial metabolism and apoptosis in CS-exposed rats and exhibits enduring cardio protection at mitochondrial level, where it shelters the activities of TCA cycle enzymes and antioxidant enzymes from CS exposure with concomitant decrease in lipid peroxidation and increase in GSH level, inhibits apoptosis through a series of cellular events, including inhibition of the release of cytochrome c into cytosol, activation of pro-caspase-3, down-regulation of Bax and up-regulation of Bcl-2, and reverses the ultra structural apoptotic alternations of mitochondria and nucleus.

2. The reconstructed skin micronucleus assay in EpiDerm™: reduction of false-positive results - a mechanistic study with epigallocatechin gallate. Mutat Res. 2013 Oct 9;757(2):148-57. doi: 10.1016/j.mrgentox.2013.08.001. Epub2013 Aug 27.Abstract EGCG, which causes positive results in in vitro mammalian genotoxicity assays via oxidative stress and negative results in in vivo MN studies, was used to assess the effect of cell differentiation status on MN induction and significantly induced the expression of genotoxic response related genes in NHEKs after 12h. The Oxidative stress probably caused by EGCG can be eliminated by EpiDermTM under in vitro experimental conditions.

3. Assessment of DNA damage and lipid peroxidation in diabetic mice: effects of propolis and epigallocatechin gallate (EGCG). Mutat Res. 2013 Sep 18;757(1):36-44. doi: 10.1016/j.mrgentox.2013.04.022. Epub2013 Jul 13.Abstract EGCG, a strong antioxidant with possible anti-obesity and anti-diabetic effects, was administered intraperitoneally to alloxan-induced diabetic mice at doses of 50mg/kg body weight for a period of 7 days resulting in a significant increase of body weight and haematological/immunological blood parameters, 100% survival, a significant decrease of lipid peroxidation in liver, kidney and brain tissue, and remarkably reduced DNA damage in peripheral lymphocytes. Due to the demonstrated beneficial effects against diabetes and the associated consequences of free-radical formation in kidney, liver, spleen and brain tissues, EGCG could be used as a dietary supplement potentially contributing to nutritional strategies for the prevention and treatment of diabetes mellitus.

4. Epigallocatechin-gallate (EGCG) regulates autophagy in human retinal pigment epithelial cells: a potential role for reducing UVB light-induced retinal damage. Biochem Biophys Res Commun. 2013 Sep 6;438(4):739-45. doi: 10.1016/j.bbrc.2013.07.097. Epub2013 Jul 31.Abstract EGCG is involved in UVB irradiation-induced autophagy in RPE cells, where the treatment of EGCG significantly reduced the formation of LC3-II and autohagosomes and remarkably relieved UVB irradiation-induced toxicity to RPE cells in an autophagy-dependent manner, and is a potential therapeutic reagent that could be incorporated into the treatment of pathological conditions associated with abnormal autophagy.

5. Green tea epigallocatechin gallate binds to and inhibits respiratory complexes in swelling but not normal rat hepatic mitochondria. Biochem Biophys Res Commun. 2014 Jan 17;443(3):1097-104. doi: 10.1016/j.bbrc.2013.12.110. Epub2013 Dec 31.Abstract EGCG, which is hepatotoxic to humans and animals, barely affected oxidative phosphorylation at 7.5-100 μΜ in normal mitochondria of rats liver but remarkably inhibited RCCs in mitochondria undergoing Ca(2+) overload-induced MPT only if IM integrity was compromised. EGCG promotes Ca(2+) overload-induced MPT after moderate MPT has already commenced and worsens pre-existing mitochondria abnormalities triggering hepatotoxicity since it only targets hepatic RCCs in swelling mitochondrial other than normal mitochondrial.

Background

(-)-Epigallocatechin gallate (EGCG), the major catechin accounting for 59% of the total catechins in green tea, is a powerful antioxidant as well as an antiangiogenic and antitumor agent. EGCG has been studied for its role in the chemoprevention of a wild range of cancers, including liver, stomach, skin, lung, mammary gland and colon cancers. Study results show that EGCG is able to induce apoptosis, promote cell growth arrest and block carcinogenesis by affecting signal transduction pathways. Moreover, EGCG exhibits inhibition against a variety of viruses, including HCV, HIV-1, HBV, HSV-1, HSV-2, EBV, adenovirus, influenza virus and enterovirus, as well as several enzymes, including DNMTs, proteases and DHFR.

Reference

Singh BN, Shankar S, Srivastava RK. Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochem Pharmacol. 2011; 82(12):1807-1821.

Steinmann J, Buer J, Pietschmann T, Steinmann E. Anti-infective properties of epigallocatechin-3-gallate (EGCG), a component of green tea. Br J Pharmacol. 2013; 168(5):1059-1073

品牌介绍

ApexBio是用于生物学研究的肽和实验室试剂的主要提供商。我们在细胞凋亡，表观遗传学，癌症生物学等研究领域拥有广泛的产品线。Apexbio还提供定制服务，包括肽合成，抗体生产和测定开发。

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