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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ApexBio/EZLink NHS-Biotin Kit/10 reactions/K1002
产品编号:K1002
市  场 价:¥4320.00
场      地:美国(厂家直采)
产品分类: 蛋白类>聚合物>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$216.00
品      牌: ApexBio
公      司:ApexBio
公司分类:
ApexBio/EZLink NHS-Biotin Kit/10 reactions/K1002
商品介绍
Related Products
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EZLink NHS-Biotin KitAmine-reactive biotinyltation kit

Catalog No.K1002
SizePriceStockQty
10 reactions
$216.00
In stock

Tel: +1-832-696-8203

Email: sales@apexbt.com

Worldwide Distributors

Sample solution is provided at 25 µL, 10mM.

Publications citing ApexBio Products

Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.
Nature.2018 Jun 13.
Nature.2018 Jun 27.
Nature.2018 Mar 29;555(7698):673-677.
Nature.2017 Sep 7;549(7670):96-100.
Nature.2016 Apr 21;532(7599):398-401.
Science.2016 Aug 5;353(6299)594-8
Nat Nanotechnol.2017 Dec;12(12):1190-1198.
Nature Biotechnology.2017 Jun;35(6):569-576
Nat Med.2018 Sep 17.
Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.
Cell.Available online 25 October 2018.
Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.
Cell.2018 Jun 28;174(1):172-186.e21.
Cell.2018 Feb 22;172(5):1007-1021.e17.
Cell.2017 Nov 30;171(6):1284-1300.e21.
Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.
Cell.2017 Jul 13;170(2):312-323
Nat Med.2018 Jan 29.
Nat Med.2017 Nov;23(11):1342-1351.
Cell.2017 Apr 6;169(2):286-300.
Cell.2015 Aug 27;162(5):987-1002.
Cell.2015 Feb 12;160(4):729-44.
Nature Medicine.2017 Apr;23(4):493-500.
Cancer Cell.2018 May 14;33(5):905-921.e5.
Cancer Cell.2018 Apr 9;33(4):752-769.e8.
Cancer Cell.2018 Mar 12;33(3):401-416.e8.
Cancer Cell.2017 Aug 14;32(2):253-267.e5.
Nat Methods.2018 Jul;15(7):523-526.
Cell Stem Cell.2018 May 3;22(5):769-778.e4.
Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.

Components and storage

Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
Streptavidin10 mgEZ-Link NHS-Biotin25 mg
HABA Solution1 mLPBS Pack (makes 500 mL)1 pack
Sephadex G-25 in PD-10 Desalting Columns8.3 mL, 10 columns
Store biotin and streptavidin reagents at -20°C. Store remaining kit components at 4°C.

Specification

Label or Dye:BiotinProduct Size:10 reactions
Labeling Scale:1-10 mgLabeling Target:Proteins (General), Antibodies (General)

Description

The EZLink NHS-Biotin Kit contains reagents sufficient for 10 biotin labeling reactions. It is a water-soluble biotin reagent for labeling protein, antibody or other molecules with amidogen (NH2-). The biotin in the kit is sufficient and has been activated for direct use. The whole procedure is simple and quick.

Advantage

Accurate Ingredients:

Clearance of components and concentrations.

Higher efficiency:

Quick achievement, quality assurance.

Lower price:

Our price is less than any of competitor.

Specialty

• Amine-reaction—Reacting with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides.• Labeling antibody—This kit can label antibodies to facilitate immobilization, purification or detection.• Labeling protein—This kit can label proteins to facilitate immobilization, purification or detection.• Labeling Cell surface molecules—This kit can label the cell surface proteins because the negatively charged reagent does not permeate cell membranes.• Irreversible-Permanent amide bonds are formed; Spacer arm cannot be cleaved.• Very short arm—Spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.• Solubility increased—Sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds.

NHS-Biotin (C14H18O5N3S) is N-hydroxysuccinimido biotin. It is the shortest of three similar EZ-Link NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. NHS-Biotin offers researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because it is uncharged and contain simple alkyl-chain spacer arms, NHS-Biotin compound is membrane-permeable and useful for intracellular labeling.NHS-Biotin is the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.

品牌介绍


ApexBio是用于生物学研究的肽和实验室试剂的主要提供商。我们在细胞凋亡,表观遗传学,癌症生物学等研究领域拥有广泛的产品线。Apexbio还提供定制服务,包括肽合成,抗体生产和测定开发。


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