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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ApexBio/ApexPrep DNA Plasmid Miniprep Kit/300 tests/A5001
产品编号:A5001
市  场 价:¥4000.00
场      地:美国(厂家直采)
产品分类: 蛋白类>聚合物>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$200.00
品      牌: ApexBio
公      司:ApexBio
公司分类:
ApexBio/ApexPrep DNA Plasmid Miniprep Kit/300 tests/A5001
商品介绍
Related Products
  • ApexPrep DNA Plasmid Miniprep Column Only
ApexPrep DNA Plasmid Miniprep Kitused for isolation of plasmid DNA

Catalog No.A5001
SizePriceStockQty
50 tests
$40.00
In stock
300 tests
$200.00
In stock

Tel: +1-832-696-8203

Email: sales@apexbt.com

Worldwide Distributors

Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Wang EX, Ding MZ, et al. "Reorganization of a syntheticmicrobial consortium for one-step vitamin C fermentation." Microb Cell Fact. 2016 Jan 25;15:21.PMID:26809519

Description

For purification ofup to 20 μg molecular biology grade plasmid DNA

  • Ready-to-use plasmid DNA in minutes
  • Reproducible yields of molecular biology grade plasmid DNA
  • Single protocol for high- and low-copy vectors

The ApexPrep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.

DNA Miniprep Protocol Using a Microcentrifuge1. Inoculate 1-5 ml LB medium (selective antibiotic added) with a single colony, incubate with vigorous shaking at 37 °C overnight. 2. Harvest bacteria by centrifuging at > 6000 rpm for 5 min. Remove supernatant and keep pellet. 3. Resuspend bacterial pellet with 250 μl Buffer P1 and transfer to a fresh microcentrifuge tube. 4. Add 250 μl Buffer P2 and mix gently by inverting 5-6 times at room temperature. 5. Add 350 μl Buffer P3 and mix gently by inverting 5-6 times. Spin down at 12000 rpm for 10 min to remove bacterial/protein debris. Transfer supernatant to a spin column. 6. Centrifugation at 6000 rpm for 1 min. Discard flow-through.7. (Optional), wash spin column with 0.5 ml Buffer PB; centrifuge at 6000 rpm for 1 min, and discard flow-through.8. Wash spin column with 0.75 ml Buffer PE; centrifuge at 6000 rpm for 1 min, and discard flow-through.9. Centrifuge at 12000 rpm for an additional 1 min to remove residual wash buffer.10. To elute DNA, place the column in a clean 1.5 ml collection tube. Add 50 μl Buffer EB to the center of the membrane, let stand for 1 min, and centrifuge at 12000 rpm for 1 min to collect eluted DNA.

品牌介绍


ApexBio是用于生物学研究的肽和实验室试剂的主要提供商。我们在细胞凋亡,表观遗传学,癌症生物学等研究领域拥有广泛的产品线。Apexbio还提供定制服务,包括肽合成,抗体生产和测定开发。


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